Abstract

Response: Is Regulation of a Chloride Channel in Lymphocytes Affected in Cystic Fibrosis?

Highlights

  • Response: The comment by Hagiwara et al points out the difficulty in studying the chloride channel in epithelial cells and lymphocytes

  • The failure of Hagiwara et al, to induce chloride channel gating by cAMP-dependent phosphorylation in sweat gland cells as well as lymphocytes suggests that the same variable may be affecting results in both of their preparations

  • Note added in proof Recent evidence (1) suggests that a cAMP-dependent lymphocyte anion permeability is activated during G1 phase of the cell cycle and that this activation is not detectable in CF-derived lymphocytes

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Summary

AND NOTES

Stanford University School of Medicine, who supplied the catalytic subunit of cAMP-dependent protein kinase, which was prepared, assayed, and applied essentially as in (1). Response: The comment by Hagiwara et al points out the difficulty in studying the chloride channel in epithelial cells and lymphocytes. The failure of Hagiwara et al, to induce chloride channel gating by cAMP-dependent phosphorylation in sweat gland cells as well as lymphocytes suggests that the same variable may be affecting results in both of their preparations. Note added in proof Recent evidence (1) suggests that a cAMP-dependent lymphocyte anion permeability is activated during G1 phase of the cell cycle and that this activation is not detectable in CF-derived lymphocytes. The suggestion that the chloride channel is cell cycle-dependent may account for some of the apparent variability in channel density

Sphingomyelin Synthase and PKC Activation
Protein kinase C
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