Abstract
Response: Is Regulation of a Chloride Channel in Lymphocytes Affected in Cystic Fibrosis?
Highlights
Response: The comment by Hagiwara et al points out the difficulty in studying the chloride channel in epithelial cells and lymphocytes
The failure of Hagiwara et al, to induce chloride channel gating by cAMP-dependent phosphorylation in sweat gland cells as well as lymphocytes suggests that the same variable may be affecting results in both of their preparations
Note added in proof Recent evidence (1) suggests that a cAMP-dependent lymphocyte anion permeability is activated during G1 phase of the cell cycle and that this activation is not detectable in CF-derived lymphocytes
Summary
Stanford University School of Medicine, who supplied the catalytic subunit of cAMP-dependent protein kinase, which was prepared, assayed, and applied essentially as in (1). Response: The comment by Hagiwara et al points out the difficulty in studying the chloride channel in epithelial cells and lymphocytes. The failure of Hagiwara et al, to induce chloride channel gating by cAMP-dependent phosphorylation in sweat gland cells as well as lymphocytes suggests that the same variable may be affecting results in both of their preparations. Note added in proof Recent evidence (1) suggests that a cAMP-dependent lymphocyte anion permeability is activated during G1 phase of the cell cycle and that this activation is not detectable in CF-derived lymphocytes. The suggestion that the chloride channel is cell cycle-dependent may account for some of the apparent variability in channel density
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