Abstract
Summary Antibodies produced by immunization of rabbits with Shigella flexneri 1b and 2a were demonstrated in both the IgM and IgG fractions of the serum. A procedure for fractionation of antibodies by salting out followed by gel filtration and diethylaminoethyl column chromatography was found to be the best method for the purification and subfractionation of IgM and IgG from rabbit antiserum. Studies on the specificities of the two types of immune globulins demonstrated a high specificity for the IgG and a low specificity for the IgM. The subfractions derived from each type, however, demonstrated almost the same reactive pattern notwithstanding that they were slightly different from each other chromatographically and electrophoretically. The antibody pattern for type specific antigen I was predominant in IgM, and that for group antigen 6 was predominant in IgG of the anti-Shigella flexneri 1b serum. The possible mechanism for the different specificities of the two types of antibodies was discussed.
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