Response and oil degradation activities of a northeast Atlantic bacterial community to biogenic and synthetic surfactants

Christina N Nikolova,Tony Gutierrez,Umer Zeeshan Ijaz,Samantha B Joye,Sara Kleindienst,Clayton Magill

doi:10.1186/s40168-021-01143-5

Christina N Nikolova, Tony Gutierrez + Show 4 more

Open Access

https://doi.org/10.1186/s40168-021-01143-5

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Abstract

BackgroundBiosurfactants are naturally derived products that play a similar role to synthetic dispersants in oil spill response but are easily biodegradable and less toxic. Using a combination of analytical chemistry, 16S rRNA amplicon sequencing and simulation-based approaches, this study investigated the microbial community dynamics, ecological drivers, functional diversity and robustness, and oil biodegradation potential of a northeast Atlantic marine microbial community to crude oil when exposed to rhamnolipid or synthetic dispersant Finasol OSR52.ResultsPsychrophilic Colwellia and Oleispira dominated the community in both the rhamnolipid and Finasol OSR52 treatments initially but later community structure across treatments diverged significantly: Rhodobacteraceae and Vibrio dominated the Finasol-amended treatment, whereas Colwellia, Oleispira, and later Cycloclasticus and Alcanivorax, dominated the rhamnolipid-amended treatment. Key aromatic hydrocarbon-degrading bacteria, like Cycloclasticus, was not observed in the Finasol treatment but it was abundant in the oil-only and rhamnolipid-amended treatments. Overall, Finasol had a significant negative impact on the community diversity, weakened the taxa-functional robustness of the community, and caused a stronger environmental filtering, more so than oil-only and rhamnolipid-amended oil treatments. Rhamnolipid-amended and oil-only treatments had the highest functional diversity, however, the overall oil biodegradation was greater in the Finasol treatment, but aromatic biodegradation was highest in the rhamnolipid treatment.ConclusionOverall, the natural marine microbial community in the northeast Atlantic responded differently to crude oil dispersed with either synthetic or biogenic surfactants over time, but oil degradation was more enhanced by the synthetic dispersant. Collectively, our results advance the understanding of how rhamnolipid biosurfactants and synthetic dispersant Finasol affect the natural marine microbial community in the FSC, supporting their potential application in oil spills.43fSDwbdHYX3Ei4X1BHK1YVideo abstract

Highlights

Biosurfactants are naturally derived products that play a similar role to synthetic dispersants in oil spill response but are biodegradable and less toxic
We investigated whether the presence of a rhamnolipid and the synthetic dispersant Finasol OSR52, which is stockpiled worldwide for use in oil spill response, would result in a distinct shift in the taxonomic composition of a natural marine microbial community from the Faroe-Shetland Channel (FSC) and which taxa would be more likely responsible for the compositional shifts over time
Local Contribution to Beta Diversity (LCBD) analysis showed that diversity varied with time in all treatments but by the end of the incubation period, the community assembly in the Chemically Enhanced WAF (CEWAF) and seawater and Finasol (SWD) treatments were markedly different from the average community structure (Supplementary Figure S1)

Summary

Introduction

Biosurfactants are naturally derived products that play a similar role to synthetic dispersants in oil spill response but are biodegradable and less toxic. Extensive tracking of the microbial response to crude oil contamination in the ocean after the Deepwater Horizon (DWH) oil spill in the Gulf of Mexico in 2010 provided an unprecedented view into feedbacks between environmental chemical signatures and microbial community evolution [1]. During this historic spill, approximately 700,000 tonnes (4.9 million barrels) of Louisiana light sweet crude oil was discharged into the Gulf from a blown-out wellhead at a depth of ~ 1500 m. Questions were raised about the response of autochthonous populations of hydrocarbon-degrading (hydrocarbonoclastic) bacteria—key players in oil biodegradation—to these dispersants, and the need to identify the impact of dispersants on oil bioremediation was highlighted

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