Abstract
Abstract: Inga vera ssp. affinis embryos at different maturity stages and with various water contents had their CO2 production and O2 consumption analyzed, and then compared to those of Phaseolus vulgaris L. seeds. The results showed that the lower the temperature, the lower the respiratory rates and the more intense the other oxidative processes of I. vera embryos. On the other hand, the same did not happen in P. vulgaris seeds. Oxidative reactions, including respiration, reduced the germinative capacity and compromised the preservation of the embryos and seeds. Oxidative reactions depended on the embryo maturity stage, its moisture level, and the surrounding temperature. Interestingly, the responses obtained for viability loss and changes in O2 consumption rates of I. vera embryos did not correspond to the expected for plant tissues, as verified in P. vulgaris seeds with similar water content.
Highlights
Recalcitrant seeds are difficult to be preserved during storage due to their sensitiveness to desiccation
The percentages of seeds able to protrude the radicle, corresponding to these stages, were 90, 97, and 90%, while the values of germination were 47, 97, and 85%, respectively. Such outcomes validate the evolution of maturation throughout the stages, as reported by Bonjovani and Barbedo (2008)
The results on O2 consumption and CO2 release of embryos incubated at -2 to 15 °C showed intense metabolic activity, probably respiratory, especially at the highest temperatures (Figure 1)
Summary
Recalcitrant seeds are difficult to be preserved during storage due to their sensitiveness to desiccation. Barbedo an essential role in the deterioration process, especially when the water content is high (Barbedo et al, 2013; Caccere et al, 2013) Keeping this intense activity, especially under conditions of high ambient temperature, demands elevated respiratory rates and the consumption of seed reserves (Araújo and Barbedo, 2017). Affinis (DC.) T.D. Pennington, known as ingá, produces seeds that are sensitive to desiccation and naturally have low longevity (Caccere et al, 2013; Lamarca and Barbedo, 2015). Pennington, known as ingá, produces seeds that are sensitive to desiccation and naturally have low longevity (Caccere et al, 2013; Lamarca and Barbedo, 2015) This species greatly interests studies aiming at the development of procedures to expand the storage potential of recalcitrant seeds. On that account, considering the lack of information about the respiratory metabolism of recalcitrant seeds, the present work aimed at assessing the O2 consumption and CO2 release rates of I. vera embryos at different levels of hydration and subjected to different temperatures of incubation, analyzing their relationship with the preservation capacity of the seeds
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