Abstract
Fluorescence quenching of dye molecules such as coumarin 153, DCM and nile red by the stable 2,4,6-tri-tert-butylphenoxyl radical in an MTHF glass matrix at 77 K was studied by means of subnanosecond time-resolved fluorescence spectroscopy. The quenching process is ascribed to the dipole-dipole interaction amenable to Förster theory. The average critical energy transfer distances were determined to be 20, 27 and 31 Å for coumarin 153, DCM and nile red, respectively. These values are consistent with those evaluated from spectroscopic data.
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