Resonance assignment of the first and second KH (hnRNP-K homology) domains of human poly(C)-binding protein-2 (PCBP2)

Abstract

PCBP2 is a ~40 kDa protein containing three copies of the KH nucleic acid binding domain. It is a member of the PCBP family that recognizes poly(C) DNA and RNA sequences in mammalian cells. Binding poly(C) sequences via the KH domains is critical for various PCBP functions, such as mRNA stability, translational activation/suppression, etc. To better understand how PCBP functions are regulated by RNA/DNA binding, we carried out NMR studies of the N-terminal KH domains of PCBP2 (residues 11-169, the NMR construct contains MKH6K prior to the native sequence). The extent of backbone and overall N, C, and H chemical shift assignments is 98 and 90% respectively. BMRB deposit with accession number 15049.