Resolution of the spectra of acetamiprid, flutolanil and etofenprox residues for their analysis in tomato fruits.

Abstract

This study involves spectroscopic analysis of pesticide residues extracted from tomato, one of the most freshly eaten fruit all over the world. In Egypt, tomato can be protected against pests infection by concomitantly spraying three pesticides namely, acetamiprid (AC), flutolanil (FL) and etofenprox (ET). The three pesticides have been simply and efficiently extracted from the fruits and analyzed by applying the following methods: Differential dual wavelength method, where AC, FL and ET were determined by amplitudes subtraction at 264.8-277nm, 229-241nm and 225.6 and 243nm, respectively after obtaining their first derivative spectra. Modified ratio difference method, where the difference in amplitude values at 261.2 and 241nm, 273.4 and 236.8nm and 269.8 and 232nm was used for determination of AC, FL and ET, respectively. The third method includes recording the amplitudes at 284, 293 and 224nm for AC, FL and ET, respectively, after mean centering of their spectra. The linear ranges were 1-11, 0.2-2.5 and 0.2-2.5μgmL-1 for AC, FL and ET, respectively. The methods were proven to be green regarding the Eco-Scale calculations. The methods were efficiently applied for determination of AC, FL and ET in their commercial forms and field trials, where the residues were approximately equal to or below their specified maximum residue limits.