Abstract
A method has been described for the resolution of polyribosomes and RNA prepared from both mammalian and bacterial sources. Although numerous studies have shown the presence of the components we have studied, our results demonstrate a unique resolution of ribosomal complexes that are closely related in size by automatic density gradient fractionation. The fractionation of RNA by this method is not appreciably improved over those which have been previously published. However, this automatic method is rapid and reproducible and presents a convenient means for the analytical determination of various complexes of ribosomes and RNA. Furthermore, the amounts resolved may be sufficient in quantity for in vitro amino acid incorporation or sRNA-ribosome binding studies.
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