Resolution of racemic acyl‐amino acids‐ 14C by water‐insoluble hog kidney acylase

Abstract

AbstractFor the purpose of continous resolution of racemic N‐acyl‐amino acids‐14C conditions for the preparation and utilization of water‐insoluble hog kidney acylase (E. C. 3. 5. 1. 14) were investigated, A stable, highly active water‐insoluble acylase was easily prepared by linking the enzyme to DEAE‐Sephadex A‐50 in Tris‐HCL‐buffer, μ 0.045; at pH 7. 1.