Abstract
The analysis of a reaction product by high-performance liquid chromatography coupled with Fourier transform spectroscopy (LC-FT-IR) originates a series of overlapping peaks. The applicability of the orthogonal projection approach, the fixed size window evolving factor analysis approach, and the evolving factor analysis approach for the resolution of those overlapping peaks into individual chromatograms and IR spectra is discussed. The results are evaluated by identifying the different compounds present using the spectral characteristics of the resolved pure compound spectra and the chemistry of the system.
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