Resolution of complex anisotropy decays by variable frequency phase-modulation fluorometry: a stimulation study

Abstract

We used simulations to determine the resolution of complex anisotropy decay laws which is obtainable by frequency-domain fluorometry. The simulations include the effects of torsional and segmental motions of tryptophan residues in proteins, the multiple correlation times of asymmetric molecules, and three-component anispotropy decays. For a protein with a global correlation time of 10 ns it should be possible to resolve torsional motions with correlation times as short as 10 ps if the amplitude of the rapid motion is at least 20% of the total anisotropy decay with r 0 = 0.4. Correlation times which differ by only 1.4-fold can be resolved, making this method useful for determination of the shape of proteins and other asymmetric molecules. It is possible to resolve three-component anisotropy decays if the overall difference among the correlation times is 30-fold. Such resolution will be useful for understanding of internal motions of proteins and membranes. The validity of these predictions is demonstrated in the subsequent paper using experimental data for melittin in solution and when bound to membranes (Maliwal, B.P., Hermetter, A. and Lakowicz, J.R. (1986) Biochim. Biophys. Acta 873, 173–181).