Abstract
Lipase from pseudomonas sp. (PSL) was encapsulated in stacked silica nanoparticles and used for resolution of 1,1,1-trifluoro-2-octanol. The effects of reaction conditions, such as solvent, temperature, water activity and type of acyl donor were investigated. Compared with the free lipase, the encapsulated lipase exhibits better performance (enzyme activity, 20.32 μmol g−1 min−1; E value, 33.83) under optimum conditions. Most importantly, almost no enzyme leakage phenomenon takes place during continuous batch operation, which suggests that this new encapsulation method has great potential for application.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.