Abstract
We have studied “static” factors affecting resolution in zonal rotors, that is, factors that are independent of diffusion or sedimentation of the sample. For most zonal rotors under the recommended conditions, there is proportionately very little zone broadening of sample volumes larger than 10 cc. There appears to be a correlation between the height of the rotor and resolution. We show that step gradients surrounding the sample can result in zone broadening in the case of slowly sedimenting particles. We did not detect any deleterious effects from increasing the initial sample radius.
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