Abstract
Direct-method phase extension has been applied to two-dimensional electron diffraction data of the protein streptavidin. Structure-factor amplitudes from electron diffraction were combined with phases from the corresponding electron micrographs. Maximum-entropy discrimination and cluster analysis were used to derive a solution from a large number of random trials. The phase extension from 0.3 to 0.25nm led to substantial improvement of the reconstructed projection image quality.
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