Abstract
Integral-imaging technology has demonstrated its capability for computing depth images from the microimages recorded after a single shot. This capability has been shown in macroscopic imaging and also in microscopy. Despite the possibility of refocusing different planes from one snap-shot is crucial for the study of some biological processes, the main drawback in integral imaging is the substantial reduction of the spatial resolution. In this contribution we report a technique, which permits to increase the two-dimensional spatial resolution of the computed depth images in integral microscopy by a factor of √2. This is made by a double-shot approach, carried out by means of a rotating glass plate, which shifts the microimages in the sensor plane. We experimentally validate the resolution enhancement as well as we show the benefit of applying the technique to biological specimens.
Highlights
Three-dimensional (3D) live imaging is important for a better understanding of some biological processes
In this paper we propose to enhance the lateral resolution of integral microscope (IMic) by means of a physical interpolation of two shifted radiance maps
In order to improve the resolution performance of the IMic, we propose a method based on the physical interpolation of two shifted radiance maps
Summary
Three-dimensional (3D) live imaging is important for a better understanding of some biological processes. Computing this function from the captured microimages, orthographic views and depth images can be reconstructed Taking these characteristics into account, InI is a promising technique when applying it to microscopy, especially when there is the need for recording high-speed volumetric information. An interpolation procedure over the micro-images has been reported [22], which, it improves the visual aspect, does not increase the resolution as this is limited by the previously existing information This kind of microscopy has been named in different ways, like lightfield microscopy, or plenoptics microscopy, we would like to acknowledge the importance of Lippmann in the inception of this technology and coin the name integral microscopy.
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