Abstract
A method for improving lateral resolution and reducing imaging distortion of optical subtraction microscopy is proposed. First, an azimuthally polarized (AP) light is modulated by an annular aperture. Then, an image with higher lateral resolution is obtained by subtracting an image obtained by an annular AP beam from an image obtained by a radially polarized beam. The simulation results demonstrate that compared with the case without the annular aperture, negative side-lobes in the effective point spread function are effectively suppressed, and the imaging quality of subtraction microscopy is obviously enhanced.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.