Abstract
Resistin is an adipokine produced in white adipose tissue that is thought to modulate insulin sensitivity in peripheral tissues (such as liver, skeletal muscle or adipose tissue). Human and murine resistin molecules share only about 60% sequence homology. [1] Contrary to humans, in which resistin is secreted mostly by macrophages, Park and Ahima 2013 resistin in rodents is produced primarily by the mature adipocytes of the white adipose tissue. Although resistin can bind to toll-like receptor 4 (TLF4) activating proinflammatory responses in human and rodents, [3–8] the inflammatory actions of resistin in human monocytes were found to be mediated by resistin binding to adenylyl cyclase-associated protein 1 (CAP1). [9] In this study, we aimed to investigate the in vitro effects of resistin on the expression of various genes related to insulin resistance in mouse liver cells. Using BNL CL.2 cells, we investigated the effect of resistin in untransfected or CAP1 siRNA-transfected cells on the expression of 84 key genes involved in insulin resistance.
Highlights
Resistin and adenylyl cyclase-associated protein 1 (CAP1) regulate the expression of genes related to insulin resistance in BNL CL.2 mouse liver cells
Resistin can bind to toll-like receptor 4 (TLF4) activating proinflammatory responses in human and rodents, [3e8] the inflammatory actions of resistin in human monocytes were found to be mediated by resistin binding to adenylyl cyclase-associated protein 1 (CAP1)
Using BNL CL.2 cells, we investigated the effect of resistin in untransfected or CAP1 siRNAtransfected cells on the expression of 84 key genes involved in insulin resistance
Summary
Related research article Lee S, Lee HeC, Kwon YeW, Lee SE, Cho Y, Kim J et al Adenylyl cyclase-associated protein 1 is a receptor for human resistin and mediates inflammatory actions of human monocytes. Value of the data The role of murine resistin in mediating insulin sensitivity of the peripheral tissues and adenylyl cyclase-associated protein 1 (CAP1) as potential resistin receptor is not yet well understood. We used BNL CL. mouse liver cells that were treated with resistin in the presence or absence of CAP1 siRNA transfection. We found that resistin modulates gene expression of several genes related to insulin resistance, and the effect of some of these genes is modulated by CAP1
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