Abstract
The aims of study to describe the resistance of water spinach (Ipomoea aquatica (L.) Buch) for remediation of lead polluted water, based on rate growth rate (RGR), lead content in plant, and percentage of free amino acids. Plants of equal size was grown hydroponically and exposed to 0, 1, 5, 10 mgL-1 of lead concentration for 10 days, 20 days, and 30 days. Design of experiment used a completely randomized design with 3 replications. Content of lead in each plant tissues was analyzed by Inductively Coupled Plasma (ICP) and free amino acids was analyzed by HPLC (High Pressure Liquid Chromatography). Data of lead content in plant were analyzed by Anova, while free amino acids was analyzed by descriptive statistic. The results show that water spinach could grow well in lead polluted water. The bioaccumulation factor of plant were >1 with the translocation factor <1 indicated that plant are resistant to lead. This resistance is proved by the increasing of the total protein 0.60; 0.76; 0.79; and 0.94. These three free amino acids indicate the phytochelatin which is detected glutamic acid, cystine and glycine.
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