Abstract
Nicotiana repanda has resistance to many important tobacco diseases, but no tobacco cultivars are currently available that carry resistance genes derived from this species. In order to transfer resistance genes into cultivated tobacco (N. tabacum), N. repanda was first hybridized with N.sylvestris, a species that is cross-compatible with both N. tabacum and N. repanda. This N. repanda × N. sylvestris (R×S) hybrid was then hybridized with N. tabacum cv. NC2326 by protoplast fusion. Parental lines were transformed for resistance to two antibiotics, hygromycin or kanamycin, with Agrobacterium tumefaciens transformation vectors (...)
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