Abstract
SALT1 has reported resistance to frost and to being frozen in several species of arthropods. He has also reported on the synthesis and accumulation of a low molecular weight solute (glycerol) in insects and has suggested that this may explain resistance to cold2. Lozina-Lozinsky3 stated that in the caterpillar, Pyrausta nubilalis, which can tolerate extremely low temperatures, the proteins and nucleoproteins can resist an increase in concentrations of the electrolytes. Asahina and Tanno4 found that the overwintering pre-pupal larvae of a sawfly, Trichiocampus populi, were able to survive freezing at −30° C for a full day, and withstand liquid nitrogen temperature (−195.8° C) provided they had been previously frozen at temperatures lower than −20° C.
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