Resistance to cerebral ischemia/reperfusion injury in the Arctic Ground Squirrel (Urocitellus parryii) does not depend on the hibernation season

Abstract

Event Abstract Back to Event Resistance to cerebral ischemia/reperfusion injury in the Arctic Ground Squirrel (Urocitellus parryii) does not depend on the hibernation season Kelly L. Drew1* and Jeanette T. Moore1 1 University of Alaska Fairbanks, Alaska Basic Neuroscience Program, Institute of Arctic Biology, United States Each year, 15 million people suffer stroke worldwide. Of these, 5 million die and another 5 million are permanently disabled. The arctic ground squirrel (AGS; Urocitellus parryii) resists neuronal injury following cerebral ischemia/reperfusion (I/R) in vivo or following I/R modeled by oxygen glucose deprivation (OGD) in vitro. Resistance to cerebral I/R is evident at tissue temperatures of 35-37°C even when animals are not hibernating. Resistance to I/R injury in other organs and in other hibernating species have been shown to depend on the hibernation season. Resistance to cerebral I/R injury in summer AGS in vivo has suggested that the unique phenotype expressed during the hibernation season may not be necessary for cerebral I/R tolerance in AGS. Thus, we hypothesized that acute physiological events leading to cerebral I/R injury are attenuated by mechanisms independent of the hibernation season in AGS. A novel microperfusion device was used to investigate the role of the hibernation season to resistance to OGD-induced cell death in acute hippocampal slices from AGS. Hippocampus from adult AGS, was sampled during summer and winter animals. In winter, tissue from torpid animals was sampled during torpor (T0), or at 4h (T4) or 20h (T20) after inducing arousal by gentle handling and movement to an ambient temperature of 20°C. All groups were compared to Sprague Dawley rat as a positive control species vulnerable to cerebral I/R injury. Hippocampal slices (400μm) were placed into microchambers with perfusion solutions delivered at 7 μL/min and perfusate collected at 15 minute intervals. LDH in the perfusate was quantified and normalized to total LDH per slice as a measure of I/R injury. In response to 30 minutes OGD followed by reperfusion with normoxic artificial cerebral spinal fluid, acute slices from rats exhibited a 6 fold increase in LDH over baseline levels. By contrast, in AGS over all seasons, OGD-induced an increase in LDH release of only 2 times baseline levels. The smallest LDH response was observed in slices from both hibernating AGS (T0) and summer euthermic AGS. A slightly greater LDH response was observed in slices from AGS during induced, interbout arousal that was about 3 times baseline. Results suggest that resistance to cerebral ischemia does not depend on the hibernation season. Acknowledgements Support Provided: USAMRMC # 05178001, NS041069-06, P20GM103395 Disclosures: None Keywords: ground squirrels, hypoxia tolerance, ischemia reperfusion, torpor Conference: Tenth International Congress of Neuroethology, College Park. Maryland USA, United States, 5 Aug - 10 Aug, 2012. Presentation Type: Plenary Address (including special lectures) (Note, these individuals have already been invited) Topic: Cellular Properties Citation: Drew KL and Moore JT (2012). Resistance to cerebral ischemia/reperfusion injury in the Arctic Ground Squirrel (Urocitellus parryii) does not depend on the hibernation season. Conference Abstract: Tenth International Congress of Neuroethology. doi: 10.3389/conf.fnbeh.2012.27.00049 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Jul 2012; Published Online: 07 Jul 2012. * Correspondence: Dr. Kelly L Drew, University of Alaska Fairbanks, Alaska Basic Neuroscience Program, Institute of Arctic Biology, Fairbanks, Alaska, 99775-7000, United States, kdrew@alaska.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kelly L Drew Jeanette T Moore Google Kelly L Drew Jeanette T Moore Google Scholar Kelly L Drew Jeanette T Moore PubMed Kelly L Drew Jeanette T Moore Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.