Resistance status of Myzus persicae to pesticide and its relationship with enzymes

Abstract

AbstractThis study determined the resistance to five different insecticides, and assessed the change in enzymes in three Myzus persicae populations in Liuyang, Guiyang, and Longshan tobacco (Nicotiana tabacum L.)‐growing areas in Hunan province, China. The activities of the detoxification, protective, and digestive enzymes were determined using biochemical analysis. Results indicated that the Liuyang population had high resistance to Imidacloprid, and moderate resistance to Aacetamiprid and Cyhalothrin. The Longshan population showed low resistance to Phoxim, while the Liuyang and Guiyang populations both exhibited reduced susceptibility. All three populations exhibited reduced susceptibility to Methomyl. Field populations exhibited higher activity of detoxification enzymes [acetylcholinesterase (AchE), carboxylesterase (CarE), glutathione‐S‐transferase (GST), and mixed‐function oxidase (MFO)], protective enzymes (superoxide dismutase [SOD], catalase [CAT], and peroxidase [POD]), and digestive enzymes (amylase [AMS]) than the susceptible population. The Liuyang population showed the highest detoxification, protective, and digestive enzyme activity of all aphid populations. The AchE activity and resistance level to acetamiprid was significantly, positively correlated. The CarE, CAT, and AMS activity and resistance to Imidacloprid were also significantly, positively correlated. The correlation between enzyme activity and resistance to methomyl was negative, except for AchE. When CarE, CAT, and AMS activity was up to 60, aphids exhibited low resistance to Imidacloprid, and when AchE activity was up to 90, aphids exhibited medium resistance to Imidacloprid. These results provide the theoretical basis for predicting regional resistance of M. persicae to Imidacloprid and Acetamiprid, which can be used to delay evolution of resistance of M. persicae to insecticides in the field.