Abstract
BackgroundClass B metallo-β-lactamases (MBLs) can hydrolyze commonly used β-lactam-based antibiotics. The objective of this study was to determine the frequency of carbapenem resistance genes coding for MBLs in Enterobacteriaceae isolates. MethodsEnterobacteriaceae were isolated from clinical specimens from patients referring to clinical centers of Isfahan, Iran, since July 2018 to November 2019. Antibiotic susceptibility was determined by the E-test method. Also, the minimum inhibitory concentrations (MICs) of meropenem and imipenem were calculated by the E-test method in carbapenem-resistant isolates. Then, the frequency of multi-drug resistance (MDR) Enterobacteriaceae isolates was determined. Prevalence of the MBLs genes was investigated using PCR and enterobacterial repetitive intergenic consensus (ERIC)-PCR was done to determine the clonal relatedness of the isolates. ResultsTotally, 215, 70, 28, and 18 Escherichia coli, Enterobacter spp., Citrobacter spp. and Serratia spp. were isolated from 3500 clinical specimens, respectively. The isolates were collected from wound and abscess, respiratory samples, CSF, blood, and urine.The rate of carbapenem non-susceptible isolates of E. coli, Enterobacter, Citrobacter, and Serratia were as follows, respectively: 9 (4.2%), 4 (5.7%), 1 (3.6%), and 1 (5.6%). Multi-drug resistance (MDR) frequency among E. coli, Enterobacter, Citrobacter and Serratia isolates was 59.5%, 44%, 53.5%, and 55.5%, respectively. Moreover, blaNDM-1, blaIMP-1, and blaVIM-1 genes were detected in three E. coli isolates. Nine non-susceptible E. coli and 4 non-susceptible Enterobacter isolates were categorized into two main clusters according to the results of ERIC-PCR. ConclusionAccording to the results of the present study systematic surveillance to detect MβL producing bacteria and rational prescription and use of carbapenems could be helpful to prevent the spread of carbapenem resistance.
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