Resistance Phenotypes of Pseudomonas Aeruginosa Strains of Human Origin in Β – Lactamases

Abstract

The use of I² - lactam antibiotics is of utmost importance in medical practice. The natural resistance of Ps. aeruginosa strains to I²-lactamases is important for clinical reasons, however, acquired resistance is very common (Pilly et al., 2006). Acquired resistance comprises either enzymatic mechanisms or non-enzymatic ones (Strateva T et al., 2009)The aims it is to identifying I²-lactam profiles of resistance to Ps. aeruginosa strains and the last objective of this study is to evaluate the reliability and accuracy of the VITEK-2 Compact (Biome´rieux) automated identification and susceptibility testing system.The batch studied included 793 multi-resistant strains of Ps. aeruginosa, which were isolated from the Institute of Cardiovascular Diseases in IaE™i during 2013 - 2014, the isolates being taken from sputa, urine, pus and blood. Those isolates have passed susceptibility test that resistant to one or more antibiotic from third generation cephalosporin and/or resistant to aztreonam and/or intermediate or resistant to one or more antibiotic from carbapenem class.The results were interpreted with the help of the Vitek2 system, 61 beta-lactam resistance profiles being defined. Sputum is the primary source of isolates with almost 60%, the rest are from other sources i.e. urine, pus, blood, etc.As a result of the investigations we carried out, out of the total of 793 strains isolated and identified as multi-resistant Ps. aeruginosa, 61 strains were confirmed as having beta-lactam resistance profile. The result of this study confirmed that anyone could be infected by I²- lactams resistance profile regardless their age, gender, and clinical manifestation.