Resistance of Simmental Cattle Semen to Time Exposure at Room Temperature

Abstract

This research aimed to determine the resistance of Simmental cattle semen with different lifting times at room temperature. It had a completely randomized design (CRD) with seven treatments and five replications. The treatments involved lifting clotted semen at different times from the surface of liquid nitrogen at room temperature: P0 (Control), P1 (straw with lifting time of 5 seconds), P2 (straw with lifting time of 10 seconds), P3 (straw with lifting time of 15 seconds), P4 (straw with lifting time of 20 seconds), P5 (straw with lifting time of 25 seconds), and P6 (straw with lifting time of 30 seconds). Observed variables were motility, percentage of life, and sperm abnormalities. Analysis of variance was used to analyze the data. Duncan's multiple range test determined the significant effect. The results showed that the placement of Simmental cattle clotted semen at room temperature with different lifting times significantly influenced the motility and percentage of sperm life (P < 0.05), but it did not impact sperm abnormality significantly (P > 0.05). The conclusion is that the placement of Simmental cattle-clotted semen up to 20 seconds under a room temperature (28-30℃) was still feasible and a favorite for artificial insemination. The novelty of this research is to find the right and appropriate time for the placement of frozen semen for Simmental cattle so that the percentage of motility and survival of spermatozoa does not change or the resistance of frozen semen of Simmental cattle is still well maintained, making it easier for inseminators perform livestock insemination in the field conditions.