Abstract
Addition of perchloric acid (6.4% w/v final concentration) to horse α 1-proteinase inhibitor or to horse plasma neither precipitated nor inactivated α 1-proteinase inhibitor. None of the isoinhibitors of α 1-proteinase inhibitor was altered by dilute perchloric acid. This unexpected behavior led to a simplified procedure for the purification of horse α 1-proteinase inhibitor, consisting of removal of the bulk of plasma proteins, by perchloric acid precipitation and by gel filtration on Sephadex G-75 and G-200. The resulting preparations of α 1-proteinase inhibitor were immunogenically pure. The simplified purificaton procedure permitted the immunochemical comparison of the isoinhibitors of α 1-proteinase inhibitor, which proved to be immunologically identical.
Published Version
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular Enzymology
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