Abstract
The membrane potential and input resistance of mouse astrocytes in primary cultures were measured with two-channel microelectrodes. It was found that 30 mM sodium lactate (no pH o change) caused a hyperpolarization of 5 mV. A stepwise reduction in pH via HCl addition had no effect at pH o 6.5, at pH o 5.5 there was a 5mV depolarization and at pH o 4.5 the membrane depolarized by 28 mV and the input resistance increased by 4.9-times. After 20 min this change was still reversible. If the pH o was lowered by the addition of lactic acid, a pH o of 4.5 (30 mM lactic acid) caused a 45 mV depolarization and at 4.3-times increase in input resistance. Exposure to these conditions caused inversible effects. The exposure time for a 50% recovery of the membrane potential was 15 min. The results show that external acidification (for a period of 20 min) is only irreversible if lactate is present, probably due to excessive H + shifts into the cells via the H +-lactate carrier. The results are of significance to explain glial damage during incomplete ischemia.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
