Abstract

Two sets of arsenic resistance genes were isolated from the highly arsenic-resistant Leptospirillum ferriphilum Fairview strain. One set is located on a transposon, TnLfArs, and is related to the previously identified TnAtcArs from Acidithiobacillus caldus isolated from the same arsenopyrite biooxidation tank as L. ferriphilum. TnLfArs conferred resistance to arsenite and arsenate and was transpositionally active in Escherichia coli. TnLfArs and TnAtcArs were sufficiently different for them not to have been transferred from one type of bacterium to the other in the biooxidation tank. The second set of arsenic resistance genes conferred very low levels of resistance in E. coli and appeared to be poorly expressed in both L. ferriphilum and E. coli.

Highlights

  • Source or referencePlasmids pEcoR252 pBluescript SK pUC19 pGEM-T pGL10 pMC1403 pKK223-3 pSa pEcoBlunt pLfTnArs pLfArs pLfTnpUC1 pLfTn1, pLfTn2

  • Processes for the biooxidation of gold-bearing arsenopyrite concentrates were developed in the 1980s and are used in several countries [17]

  • One set is located on a transposon, TnLfArs, and is related to the previously identified TnAtcArs from Acidithiobacillus caldus isolated from the same arsenopyrite biooxidation tank as L. ferriphilum

Read more

Summary

Source or reference

Plasmids pEcoR252 pBluescript SK pUC19 pGEM-T pGL10 pMC1403 pKK223-3 pSa pEcoBlunt pLfTnArs pLfArs pLfTnpUC1 pLfTn1, pLfTn2. Apr; EcoRI inactivation cloning vector Apr lacZЈ; ColE1 replicon, cloning vector Apr lacZЈ; ColE1 replicon, cloning vector Apr; T-tailed PCR product cloning vector Kmr; RK2/Rp4 replicon, cloning vector Apr; promoterless lacZY operon, ColE1 replicon Apr; Ptac, ColE1 replicon, cloning vector Kmr Cmr Spr; IncW replicon, mobilizing plasmid Apr; pEcoR252 blunted at the BglII site and relegated to inactivate the EcoRI endonuclease Apr; from the L. ferriphilum Fairview gene bankd Apr; from the L. ferriphilum Fairview gene bankd Apr; 13-kb HindIII fragment from pLfTnArs cloned into pUC19 Asr Kmr; transposon transconjugants isolated from the mating experiment performed in E. coli Apr; 380-bp PCR product of arsR obtained using LfArsRF/LfArsRR primers, cloned into pKK223.3 Apr; 830-bp PCR product of arsRC obtained using LfArsRF/LfArsRCR primers, cloned into pKK223.3 Kmr; 1.4-kb SphI-PvuI fragment from pKK223.3 in pGL10 Kmr; 1.6-kb BamHI-PvuI fragment from pKKLfArsR in pGL10 Kmr; 2.1-kb BamHI-PvuI from pKKLfArsRC in pGL10 Apr; 350-bp PCR product of arsR promoter obtained with LfArsRLacZF/

This study
Findings
CBS domain

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.