Abstract
Resistance of grape Botrytis cinerea was analyzed based on PCR and sequencing technology, which provided a reasonable basis for guiding the species and usage of grape Botrytis cinerea. 104 monospora strains of grape B. cinerea were collected from the main grape-producing areas of China. After isolation and purification, the grape B. cinerea DNA was extracted, and PCR primers were designed according to the resistance mechanism of pyrimethene to pyrimethamines, pyridamines and diformolines. Sequence analysis showed that the resistance frequency of B. cinerea to pyrimidine was 22.22%-62.5%, the high-resistant strain frequency was 44.23%, and the mid-temperature zone was 62.5%. The total resistance rate of B. cinerea to pyrimidine was 42.60%. The resistance rate of B. cinerea to pyrimidine was 42.60%. Resistance frequencies of grape gray mold strains were significantly different. The resistance frequencies of grape gray mold strains to pyridimide were significant. The highest resistance frequencies of grape gray mold strains were 53.42% in the cool temperature zone. Only 3.85% of grape gray mold strains showed resistance to pyridimide. Therefore, the resistance of Grape B. cinerea to pyrimidine and pyrimidine is relatively common, and the resistance to enoylmorpholine is still in the initial stage.
Highlights
Introduction fungusMycelium brown, septate, conidial peduncleGrape is the second most productive fruit in the slender, gray-black, size of (960-1200)μm×(16-world
Hongmei Zhang and Huimin Jiang / Resistance Analysis of Grape Botrytis cinerea based on PCR, 2021, 67(6): 181-190 chemical agents, B. cinerea has the characteristics of frequent genetic variation, high suitability and fast growth
The results showed that the resistance frequency of B. cinerea to pyrimethanil was 22.22%-62.50%; the resistance frequency of B. cinerea to pyrimethanil in different grape-producing areas was different, the frequency of resistance in the warm temperature zone (Tianjin, Beijing area) was low, and the frequency of resistance was 22.22%
Summary
Introduction fungusMycelium brown, septate, conidial peduncleGrape is the second most productive fruit in the slender, gray-black, size of (960-1200)μm×(16-world. Grape is the second most productive fruit in the slender, gray-black, size of (960-1200)μm×(16-. 22)μm, shape like a tree, with branches, in the top of production in the world. The planting area ranks the mycelium cells expand like a sphere, around second in the world, with 100 varieties [1]. There are round, size of [8-14)] μm×[6-9]μm, sclerotia black or more than 40 kinds of diseases harming grape, such as brown, in a circular shape [4]. Staphylococcus fusiformis belongs affecting grape production [2]. B. cinerea or indirectly leading to the reduction of grape yield has strong saprophylaxis, a wide host range and can under the conditions of suitable growth of gray mold
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