Abstract
Aim: To evaluate, by confocal laser scanning microscopy (CLSM), the influence of 17% EDTA final irrigation on the penetration of an endodontic resin-based sealer into dentinal tubules after use of 2% chlorhexidine gel. Methods: Forty extracted bovine incisors were instrumented according to the groups: G1 - root canal preparation with 2% chlorhexidine gel (n=10); G2 - root canal preparation with 2% chlorhexidine gel and final irrigation with 17% EDTA (n=10); G3 - root canal preparation with saline and final irrigation with 17% EDTA (n=10); G4 - root canal preparation with saline (n=10). The samples were filled with gutta-percha using AH Plus sealer with rhodamine B fluorescent dye. After seven days, the teeth were sectioned at the coronal, middle, and apical thirds and viewed under confocal microscope. The most representative area of penetration depth was measured in each group. Statistical significance for the sealer penetration area was determined among groups using one-way ANOVA followed by Tuckey test. For thirds comparison, in each group, data were statistically analyzed using Friedman test (p<0.05). Results: The maximum penetration was provided by G2 - 2% chlorhexidine + EDTA (p=0.000). According to this criterion, no differences were found among the other groups and among thirds within the same group. Conclusions: Based on these results, the use of 17% EDTA should be indicated after root canal preparation with 2% chlorhexidine gel for smear layer removal, enhancing the AH Plus sealer penetration.
Highlights
Cleaning and shaping are considered the most important steps for the management of an infected root canal space
Endodontic sealers are used in conjunction with core filling materials in order to avoid gaps and voids
According to Mamootil and Messer[2] (2007) penetration of sealer into dentinal tubules will increase the interface between the filling material and dentin improving the sealing ability and the retention of material by mechanical locking
Summary
Cleaning and shaping are considered the most important steps for the management of an infected root canal space. A complete and threedimensional sealing of the root canal system is critical to prevent oral pathogens from colonizing and re-infecting the endodontic space[1]. Endodontic sealers are used in conjunction with core filling materials in order to avoid gaps and voids. According to Mamootil and Messer[2] (2007) penetration of sealer into dentinal tubules will increase the interface between the filling material and dentin improving the sealing ability and the retention of material by mechanical locking. Root canal filling may entomb any residual bacteria within the tubules and the chemical components of the sealer may exert an antibacterial effect[3]. Penetration of sealer into dentinal tubules is influenced by smear layer removal, by irrigation solutions and the filling technique[4,5]
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