Reserpine content of Rauvolfia verticillata callus culture elicited by Pythium sp.

Abstract

Astuti NP, Solichatun, Susilowati A. 2007. Reserpine content of Rauvolfia verticillata callus culture elicited by Pythium sp. Biofarmasi 5: 55-66. This research aimed to study the effect of elicitor derived from Pythium sp. on reserpine content of Rauvolfia verticillata (Lour.) Baillon callus culture. Callus was induced from R. verticillata’s leaf segment, and grew optimally on Murashige and Skoog’s medium with the addition of 2 mg/L NAA and 2 mg/L kinetin. Callus was elicited with elicitor derived from autoclaved Pythium sp. on the 30th day. The concentrations of elicitor were 0, 0.5, 1.0, 1.5 and 2.0 mg DW/mL. The harvesting times of elicited callus were at 0, 18, 36 and 72 hours. The morphology of callus (texture and color) was observed descriptively. The dry weight of callus and reserpine content were analyzed statistically by using General Linear Model Univariate (GLM Univariate) and followed by Duncan’s Multiply Ring Test (DMRT) at a confidence level of 95%. The results showed that the concentration of elicitor and harvesting time influenced the dry weight and reserpine content of R. verticillata callus culture significantly. As much as 2 mg DW/mL elicitor with a harvesting time at 72 hours produced the lowest dry weight of callus that was 0.039 g, but produced the highest reserpine content that was 481.900 mg/g DW of callus or increased 103.849% compared to the control.