Abstract
BackgroundAirway smooth muscle cells play a key role in remodeling that contributes to airway hyperreactivity. Airway smooth muscle remodeling includes hypertrophy and hyperplasia. It has been previously shown that the expression of CD23 on ASMC in rabbits can be induced by the IgE component of the atopic serum. We examined if other components of atopic serum are capable of inducing CD23 expression independent of IgE.MethodsSerum starved huASMC were stimulated with either IL-4, GM-CSF, IL-13, IL-5, PGD2, LTD4, tryptase or a combination of IL-4, IL-5, IL-13 each with GM-CSF for a period of 24 h. CD23 expression was analyzed by flow cytometry, western blot, and indirect immunofluorescence.ResultsThe CD23 protein expression was upregulated in huASMC in response to IL-4, GM-CSF, and IL-4/GM-CSF. The percentage of cells with increased fluorescence intensity above the control was 25.1 ± 4.2% (IL-4), 15.6 ± 2.7% (GM-CSF) and 32.9 ± 13.9% (IL-4/GMCSF combination)(n = 3). The protein content of IL-4/GMCSF stimulated cells was significantly elevated. Expression of CD23 in response to IL-4, GM-CSF, IL-4/GM-CSF was accompanied by changes in cell morphology including depolymerization of isoactin fibers, cell spreading, and membrane ruffling. Western blot revealed abundant expression of the IL-4Rα and a low level expression of IL-2Rγc in huASMC. Stimulation with IL-4 resulted in the phosphorylation of STAT-6 and an increase in the expression of the IL-2Rγc.ConclusionCD23 on huASMC is upregulated by IL-4, GM-CSF, and IL-4/GM-CSF. The expression of CD23 is accompanied by an increase in cell volume and an increase in protein content per cell, suggesting hypertrophy. Upregulation of CD23 by IL-4/GM-CSF results in phenotypic changes in huASMC that could play a role in cell migration or a change in the synthetic function of the cells. Upregulation of CD23 in huASMC by IL-4 and GM-CSF can contribute to changes in huASMC and may provide an avenue for new therapeutic options in asthma targeting ASMC.
Highlights
Airway smooth muscle cells play a key role in remodeling that contributes to airway hyperreactivity
CD23 protein expression is upregulated in huASMC by IL4, GM-CSF, or IL-4/GM-CSF Previous studies have shown that IgE immune complexes in atopic serum caused an increase in CD23 expression in Airway smooth muscle cells (ASMC) [16]
Within the huASMC stimulated by IL-4, GM-CSF or the combination of IL-4/GM-CSF, two populations of cells were detected distinguishable by cell size
Summary
Airway smooth muscle cells play a key role in remodeling that contributes to airway hyperreactivity. Airway smooth muscle cells (ASMC) may play a secretory or immunomodulatory role by producing pro-inflammatory cytokines, chemokines, polypeptide growth factors, extracellular matrix proteins, cell adhesion receptors, and co-stimulatory molecules, which perpetuate submucosal inflammation [4,5]. These mediators may act on the ASM itself in an autocrine manner as well to further contribute to the asthma phenotype [6]. Regulation of airway smooth muscle hypertrophy and migration may be a new target for treatment of asthma [7,8]
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