Research strategy of the therapeutic quality of mud and salty water from Ursu(Bear) Lake, Sovata,

Abstract

Infertility (sterility) is a health problem affecting 15% of couples of reproductive age. Today only a few are known about causes and treatment options involved in the pathology of infertility, while a number of issues remain unknown. Currently natural therapeutic factors from Sovata are used, based on experience over the years, for the following causes of infertility: tubal obstruction, laparoscopic postneosalpingostomiei recovery treatment (prevention of restenozations), sequelae after pelvic inflammatory disease, peritonitis, post‐inflammatory adhesions; secretory ovarian dysfunction (estrogen, progesterone), polycystic ovarian syndrome, endometriosis, infertility induced by stress. Clinical data suggest that peloidotherapy is useful in preparing the uterus for in vitro fertilization, but without scientific evidence. Mud and water from Bear Lake must be analyzed, both in terms of physico‐chemical, microbiological and in terms of phyto‐and zooplankton to detect organisms that grow in the lake and are structural and biological determinants of sludge role in studies on infertility. Previous studies conducted at the Institute of Balneology, Bucharest, have suggested the existence in the lake water of certain substances having similar effects on the endometrium as the female sexual hormones. These substances, with a natural source is probably the result of the presence of different species (including Artemia salina), in the plankton composition of Bear Lake water. After identifying the groups of indigenous microorganisms and plankton composition that provides therapeutic properties, is expected to obtain and characterize a new sapropelic mud extract to demonstrate, through experimental studies of physico‐chemistry, biology, microbiology, cell biology, histology, endocrinology, gynecology and molecular biology, the therapeutical capacity of a new mud extract in the infertility treatment. Are planned experiments on laboratory animals (Wistar rats / rabbits New Zealand) taking experimentally induced infections with low levels of fertility induced by various methodologies including genital swelling controlled or pharmaceutical interventions on endocrine control. Animals with induced infertility will be treated experimentally by parenteral injections with the mud extracted from Bear Lake, Sovata, and on the other hand will do similar procedures to those used now to Sovata with natural therapeutic factors. Obtaining of these animal models for infertility is a new approach to clinical trials. In this way we intend to quantify the therapeutic effects of mud extract in the the treatment of infertility. Also, in this way we can separate placebo effects from those of spa procedures and distinguish them from the therapeutic effects attributable to mud and water from Bear Lake. Clinical research will be accompanied by biomedical and molecular determinations in vitro, using this time as an experimental model the primary cultures of endometrial cells obtained by harvesting in