Abstract
In December 2019, the global outbreak of Corona Virus Disease 2019 (COVID-19) made biosafety an attractive and crucial development direction globally. Rapid, accurate and low-cost detection of pathogenic microorganisms is one of the important issue to ensure biosafety, and is the key to epidemic prevention, control and diagnosis. This review elaborately introduces the applications of biosensors based on nucleic acid sequencing, isothermal amplification and gene editing (CRISPR/Cas) in integrated portable devices. The development of these technologies offers the possibility of developing “low-cost, high-efficiency and result-visualization” integrated detection methods. It is expected to provide technology guarantees for the effective prevention and control of pathogenic microorganisms. © 2021, Science in China Press. All rights reserved.
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