Abstract
Protein is the basis of life substance and the main undertaker of life activities. The study of protein structure and the interaction between protein and other molecules is the basis of studying the origin of life and new drugs. Near-infrared spectroscopy (NIRS) has been widely used in various fields due to its advantages of rapid, accurate, non-destructive, and cost-effective. In this paper, the recent advances and application of NIRS in study on protein structure and molecular interactions are reviewed. At the same time, NIRS combined with other techniques for protein structure research is also introduced. This review demonstrates the feasibility of using NIRS for protein structure characterization and molecular interaction analysis, which not only provides a good theoretical support, but also lay the foundation for the study of protein molecular behavior, and effectively broadens the application field of NIRS.
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