Abstract

This experiment selected Taiwan lily as experimental material to study the different ages of lily to produce different explants to induce differentiation, through subculture and the optimal conditions for tissue culture outdoor refining, and then the experimental staff established Systematic Taiwan lily flower tissue culture and rapid propagation technology. Studies have shown that the optimal medium for differentiation of Taiwan lily flower scales (winter sampling) is MS+0.2 ug/L GA3+0.1 mg/L NAA+1 mg/L 6-BA; optimal Taiwan lily leaves (winter sampling) The medium for differentiation was MS + 0.1 ug / L GA3 + 0.1 mg / L NAA + 1 mg / L KT. The best scale-induced differentiation medium for Taiwan lily (spring sampling) that was not in dormancy was MS+0.1 mg/L NAA+1.0 mg/L 6-BA, and the leaf differentiation medium was MS+0.1 mg/L NAA+ 1 mg/L IBA. MS+0.2 mg/L NAA+1.0 mg/L KT was the best callus differentiation bulb culture medium, MS+0.5 mg/L NAA+0.5 mg/L 6-BA was the best induction rooting medium.

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