Research on the Preparation of Fir Bacterial Fertilizer Using Biological Material

Abstract

The azotobacter bacteria and the phosphate-solubilizing bacteria are separated and filtered from the rhizosphere soil of the fir plantation and the enzyme activity of azotobacter bacteria, the solubilizing power of phosphate-solubilizing bacteria and the characteristics of PGPR bacteria to produce IAA are measured in this paper. The results show that: there are 5 of the 16 azotobacter bacteria whose enzyme activity is greater than 150 nmol•mL-1•h-1, respectively NGJ-4, NGX-5, NGX-4, NGX-8 and NGJ-8. Py16, Py10 and Py3 own the strongest capacity to dissolve organic phosphorus, respectively 71.31 mg / L, 59.07 mg of / L and 65.14 mg / L; Pw10，Pw6 and Pw20 own the strongest capacity to dissolve inorganic phosphorus, respectively 232.0 mg/L，185.9 mg/L，172.6 mg/L. Py18，Py16 and Py3 own the strongest capacity to produce IAA and dissolve the organic phosphorus bacteria, respectively 38.80mg / L, and 37.29mg / L, and 35.79mg / L; Pw6, Pw8 and Pw21 own the strongest capacity to produce IAA and dissolve the inorganic phosphorus bacteria, respectively 45.340 mg/L, 39.340 mg/L, 27.480 mg/ L. Based on these results, the strains of NGJ-4, NGX-5 and NGJ-8 are selected to prepare the microbial compound bacterial fertilizer using Py16, Py3 and Pw6. Then the azotobacter bacteria, organic phosphate-solubilizing bacteria and inorganic phosphate-solubilizing bacteria is respectively diluted to the solution with the ratio of 15%, and then mix them with the volume proportion of 1.5:1:1 to obtain the mixed bacteria liquid; the proportion of solid carrier ash is 20%, the proportion of fermentation medium is 50%, and the proportion of the mixed bacteria liquid is 30%.