Abstract
Abstract The feasibility of using laccase to treat oily wastewater was examined. When only laccase was added to the synthetic oily wastewater, the suitable technological conditions were laccase at 3 U/mL, pH at 6.0, a temperature of 30°C, and a reaction time of 6 h for the initial oil concentration of 120 mg/L. Under those conditions, the rate of oil removal was as high as 69%. The effects of Mg2+, Mn2+, Cu2+, and Fe2+ ions in wastewater on the rate of oil removal using laccase were investigated. The results showed that Cu2+and Fe2+ ions obviously inhibited the catalytic performance of laccase under the studied concentration. On the other hand, Mg2+ and Mn2+ ions only had slight effects on the rate of oil removal for the range of concentrations studied. A 95% oil removal rate could be obtained when actual wastewaters were treated using laccase with the additive chitosan under the suitable technological conditions.
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