Abstract
The purpose of this study was to determine the potential for biosynthesis of propionic acid and vitamin B12 by Propionibacterium freudenreichii T82 in a medium containing various sources of carbon (glucose, fructose, and saccharose). These sugars are present in apple pomaces, which are the waste from the production of apple juice. Using statistical analysis design of experiments (DoE), the results allowed us to determine which sugars (carbon sources) exert the most beneficial influence on the biosynthesis of propionic acid and cobalamin. The highest production of propionic acid by the tested bacterial strain was obtained in a medium in which glucose accounted for at least 50% of the available carbon sources. Depending on the culture medium, the concentration of this metabolite ranged from 23 to 40 g/L. P. freudenreichii T82 produced the smallest amount of acid in medium in which the dominant nutrient source was saccharose. The results obtained indicated an inverse relationship between the amount of acid produced by the bacteria and vitamin B12 biosynthesis. Because of the high efficiency of propionic acid biosynthesis by P. freudenreichii T82, the prospect of using this strain to obtain propionate with the simultaneous disposal of waste materials (such as apple pomaces) which contain glucose and/or fructose is very promising.
Highlights
Bacteria of genus Propionibacterium have been traditionally divided into two groups: skin and classic
Such waste materials could include apple pomaces, which contain sugars, proteins, pectins, fiber, vitamins and organic acids, which may affect the efficiency of synthesis of propionic acid or cobalamin by Propionibacterium spp. and relatives
The aim of this study was to determine the potential for biosynthesis of propionic acid and vitamin B12 by P. freudenreichii strain T82 and apply an unconventional statistical approach to develop the optimal composition of sugars in the medium which might stimulate high production of metabolites by this strain
Summary
Bacteria of genus Propionibacterium have been traditionally divided into two groups: skin (acnes) and classic (dairy). Due to the serious environmental damage that can be caused by chemical production of propionic acid, as well as due to the rise in demand for natural and ecological food products, there is an increasing demand for the microbial production of propionic acid, along with the desirability of using waste materials This should reduce the cost of natural production of propionc acid and make it profitable and should have environmental benefits (Baumann and Westermann 2016). Such waste materials could include apple pomaces, which contain sugars (glucose, fructose, saccharose), proteins, pectins, fiber, vitamins and organic acids, which may affect the efficiency of synthesis of propionic acid or cobalamin by Propionibacterium spp. and relatives
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