Research on photoacoustic microscopy imaging based on photoacoustic transmission matrix with a digital micromirror device

Abstract

Due to the high penetrability of ultrasonic signals in scattering media, photoacoustic imaging based on photoacoustic effect is of great significance in the research of non-destructive testing and imaging of biological tissues. However, multiple light scattering will occur after the laser is incident on the scattering medium, resulting in poor imaging quality or inability to image. In this paper, we propose a novel method for wavefront shaping and photoacoustic imaging based on a photoacoustic transmission matrix. We use the photoacoustic transmission matrix method as the algorithm for wavefront shaping, and combine the superpixel method with a digital micromirror device (DMD) to modulate the amplitude and phase of the incident light, resulting in an enhancement factor of 10.75 for the photoacoustic signal. Then, we realize photoacoustic scanning imaging by moving the two-dimensional translation stage to drive the absorber above. Compared with the inability to image the initial photoacoustic signal before shaping, the shaped photoacoustic signal can obtain a clear photoacoustic image with a contrast ratio of 16.85. The wavefront shaping and photoacoustic imaging of photoacoustic signals based on photoacoustic transmission matrix method have a good prospect in the imaging of weaker photoacoustic signals.