Abstract
Oral microbiome is potentially correlated with many diseases, such as dental caries, periodontitis, oral cancer and some systemic diseases. Twin model, as an effective method for studying human microbiota, is widely used in research of relationship between oral microbiota and dental caries. However, there were few researches focusing on caries discordant twins. In this study, in vitro assays were conducted combined with 16S rRNA sequencing analysis on oral microbiota sampled from twins who presented discordant caries experience and mice model was developed as well. Results showed that oral microbiota from caries-active twin possessed higher metabolic activity and produced more lactic production. 16S rRNA sequencing analysis showed that more than 80% of family taxa could be transferred into gnotobiotic-mice. Key caries-associated genera were significantly different between twins and the same difference in genus level could be found in mice as well (p < 0.05). This study suggested that oral microbiota of twins could be distinguished from each other despite the similarities in genetic make-up, living environment, and lifestyle. The difference in microbiota was applied to develop a mice model which may facilitate the investigation of core microbiota of dental caries.
Highlights
Dental caries, a multi-factors related disease, is a major factor of irreversible tooth decay and will in further lead to pulpitis, severe toothache, and chronic infections[1]
We found that recipient mice could presented the oral microbiota of the donors and displayed the difference of oral microbiota between the donors
In comparison to the previous study proved that Streptococcus, Prevotella, Leptotrichia, Veillonella and Fusobacterium were correlated with onset and progress of caries[18], and we found significant difference between the abundance of Streptococcus, Prevotella, Leptotrichia and Fusobacterium of the twins in the present study. 3 of these taxa showed obvious difference in the mice oral cavity including Streptococcus, Prevotella and Fusobacterium. 75% of key taxa variations in host were successfully transferred into mice and they could reflect the difference between oral microbiota of the monozygotic twins
Summary
A multi-factors related disease, is a major factor of irreversible tooth decay and will in further lead to pulpitis, severe toothache, and chronic infections[1]. Previous studies claimed that core microbiota was one of the most important elements in dental caries development[4,5,6]. In vitro model was unable to completely restore and delegate inner oral environment and microbiota due to the pH, temperature, enzymes and so forth. It can only reflect the condition of biofilm in exact situation. The younger Female 23 18.82 No No Brushing twice a day, about 3 minutes Sweet and hot food 4 Medium likely to be a better model than in vitro to imitate a relatively similar oral environment of human being. Human oral microbiota-associated mouse model had developed via transplanting human saliva to mice. It successfully reconstructed human oral microbiota in gnotobiotic mice (results under review) and it can technically be used
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
