Research on Monitoring Algorithm and Induction Trigger System of New Spectrophotometer

Abstract

In the traditional laboratory engineering bacteria culture work, it is necessary to frequently sample the bacterial solution and judge the growth period by photometric method. These operations are accompanied by pollution risk and the change of culture environment. In this paper, a design scheme of illumination monitoring algorithm of new spectrophotometer based on single chip microcomputer is proposed, The absorbance value is calculated by single chip microcomputer and the threshold is preset to trigger the induction action, so that the whole process from the beginning of culture to the triggering induction of engineering bacteria can be intelligent, automatic and non-contact. The new spectrophotometer takes STM32F103 series processor as the main control chip, and the system is mainly composed of main control board and induced action trigger device. The main control board mainly includes optical density calculation, 12 LED light control, 12 action machine control and human-computer interaction module; The induced action trigger device is composed of motor, bh1750 module, monochrome light source and other parts, which can realize illuminance detection, absorbance calculation, preset absorbance value and trigger the action machine. The system automatically adjusts the working mode of the sensor through the matrix keyboard input, displays the image through a liquid crystal display, uses the bh1750 illuminance sensor module to convert the input illuminance signal into electrical signal for wired output, processes the sensor data through MCU, performs logical operation with the preset value and performs corresponding action. The main control part of the system is small, and the detection method is simpler and faster than the traditional detection. The logic operation part is cleverly designed, the trigger time of the induced action is flexible and adjustable, and there is an adjustable timer after the induced action is triggered.