Research on mechanism of novel isoflavone F11 in protecting rotator cuff injury through vascular endothelial growth factor signal pathway

Abstract

This study discusses the mechanism of novel isoflavone F11 in protecting rotator cuff injury through vascular endothelial growth factor (VEGF) signal pathway. 60 rats were assigned into sham group, RCT group, low F11 group, middle F11 group, high F11 group, and huqian pill group. The level of IL-1β, IL-6 and tumor necrosis factor alpha (TNF-α) was detected with enzyme-linked immunosorbent assay (ELISA) method. The max load was detected with MTS biomechanics experiment. The pathological form of tissue was detected with HE staining method. The expression of VEGF and basic fibroblast growth factor (BFGF) was detected with western blot and real-time reverse transcription–polymerase chain reaction (RT-PCR). The level of IL-1β, IL-6 and TNF-α in RCT group was higher than that in sham group. The max load was reduced. Their level in low F11 group, middle F11 group, high F11 group and huqian pill group was lower than RCT group with a significant difference in max load. VEGF and BFGF expression in RCT group was lower than sham group. It was increasing along with the increasing concentration of adopted F11. The level of inflammation in serum was reduced with F11. The biomechanics of bone could be increased. Thus, it could develop protecting action on rotator cuff injury through activating the level of VEGF.