Research on mechanism of curcumin carried with PLGA-DSPE-PEG hybridized nanoparticles on regulating the nuclear receptor coactivator5 presentation, oxidative stress and presentation of alfa-fetoprotein in rats with liver cancer

Abstract

This study intends to discuss the mechanism of curcumin carried with poly (lactic-co-glycolic acid)-1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethylene glycol)-2000] (PLGA-DSPE-PEG) hybridized nanoparticles on regulating the Nuclear receptor coactivator5 (NCOA5) expression, oxidative stress and level of Alfa-fetoprotein (AFP) in rats with liver cancer. Curcumin carried with PLGA-DSPE-PEG hybridized nanoparticles was prepared. These rats were divided into normal set, model set, curcumin set and set of curcumin and nanoparticles randomly. The presentation of alanine transaminase (ALT) and aspartate aminotransferase (AST) in every set was detected. The pathological change in liver tissue with hematoxylin and eosin (H&E) staining method, level of malondialdehyde (MDA) and superoxide dismutase (SOD) was detected. Presentation of AFP and NCOA5 was detected with Western Blotting assay and real-time reverse transcription–polymerase chain reaction (RT-PCR). Level of ALT, AST, MDA and AFP in normal set was the highest, but lowest in the set of curcumin and nanoparticles. The SOD presentation in the set of curcumin and nanoparticles was the highest. There was distinct surface texture, good glossiness and orderly arrangement along edge in normal set. There were grey nodular nodules, vacuole and dark-red lumps in part of live tissue in model set. There was slightly distinct liver plate texture, punctate particles and a small amount of congestion in curcumin set. There was distinct liver plate texture with orderly arrangement and a small number of inflammatory cells in set of curcumin and nanoparticles. The level of ALT and AST in rats with liver cancer was reduced by curcumin carried with PLGA-DSPE-PEG hybridized nanoparticles. The SOD activity was increased and MDA was reduced. The AFP presentation was reduced and NCOA5 expression was increased.