Abstract
<i>Cucumber green mottle mosaic virus</i> (CGMMV) is one of the most important viruses affecting Cucurbitaceae crops. It has been found all over the world. Once spread, it may cause significant loss on economic crops. During a project from 2019 to 2021, occurrence of <i>Cucumber green mottle mosaic virus</i> (CGMMV) on main leguminosae crops (<i>Pisum sativum</i>, <i>Vicia faba</i>, <i>Vigna unguiculata</i>, <i>Glycine max</i>, <i>Phaseolus vulgaris</i>) was surveyed via random sampling in Ningbo, Zhejiang, China. Leaf chlorosis, yellowing (or whitening), shrinkage, spots and deformation were observed and sampled accordingly. Altogether 47 legume leaf samples were collected from 10 locations during different seasons. Total RNA was analyzed through real-time RT-PCR. Among 141 RNA extractions (3 repetitive extractions for each sample) tested, 15 were positive (corresponding to 9 samples, Ct<35), and 126 negative. Nine positive samples were further verified by conventional RT-PCR and sequencing. BLAST analysis showed that 7 sequences were over 98% identical to MP and CP regions of CGMMV isolate from Guangdong, China (Accession: MK933286), while the other 2 were over 97% identical to MP and CP regions of CGMMV isolate from Zhejiang, China (Accession: KM873783). Among 47 samples, 9 (19.15%) were positive, and out of 141 nucleic acid extractions, 15 were positive, accounting for 10.64%. Inoculation test on legume seedlings (<i>V. faba</i> var. Beidouqixing and <i>P. sativum</i> var. Jingpindawuxu) was carried out using infectious cDNA clone of CGMMV (pCB-CGMMV). Stunted growth, leaf deformation, chlorosis, and whitening were observed on both <i>V. faba</i> and <i>P. sativum</i> at 25 dpi (days post inoculation). Symptomatic leaves were diagnosed by real-time RT-PCR, which turned out to be CGMMV positive. It is speculated that leguminous plants are alternative hosts of CGMMV under natural conditions, and can serve as "virus reservoir" when cucurbitaceae plants are rare. Background research on economic significant pests is supposed to be conducted regularly. On one hand, it contributes to control of agricultural diseases and reduction of related losses. On the other, it would provide basic data for follow-up researches and supplement information for important pests.
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