Research on in vitro propagation techniques of vine-tea plant (Ampelopsis cantoniensis (Hook. and Arn.) Planch)

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Vine-tea plant has the scientific name Ampelopsis cantoniensis (Hook. and Arn.) Planch and is a valuable herb plant in Vietnam. This study aimed to find a method to sterilize samples and nutrient medium components in each stage of shoots regeneration, rapid shoots multiplication, complete plant creation and in vitro seedling cultivation in the nursery stage of in vitro propagation techniques of vine-tea plant. Four experiments in research room and one experiment in nursery were conducted from January to December 2023 at Hue University of Agriculture and Forestry, Vietnam. Criterias of infected, alived, clean and dead samples, rate of shoot regeneration and number of shoots/samples were measured. Research results showed that the best medium to regenerate shoots from stem section carrying dormant eyes is MS + 1.5mg BAP/l + 6.5g agar/l + 30g sucrose/l. The best medium to quickly multiply shoots is MS + 1.5 mg BAP/l + 0.2 mg IBA/l + 6.5g agar/l + 30g sucrose/l. The best complete plant-creating medium is MS + 0.5 mg α-NAA/l + 6.5g agar/l + 30g sucrose/l. In the condition that the nursery has a rain cover roof, a black net reduced the light intensity by 70% and the tissue culture nursery substrate of humus soil under the forest canopy + coconut fiber powder + smoked rice husk (1:1:1) gave the best results with survival rate reached 76.67%.