Research on glucagon-like peptide-1 receptor agonist on the function and differentiation of brown adipocytes in mice

Abstract

Objective To investigate the effects of glucagon-like peptide-1 (GLP-1) receptor agonist on the function and differentiation of brown adipocytes in mice.Methods Primary brown adipocytes in mice were cultivated in vitro.Before the induction,brown adipocytes were assigned to four groups:GLP-1 10-8mol/L group,GLP-1 10-9mol/L group,L-NAME 10-3 mol/L group,and control group.Finally,adipocytes were put under morphological observation.The expression levels of genes of brown adipocytes in different groups were detected by RT-PCR.The single factor analysis was used for data analysis.Results The lipid droplets of brown adipocytes in GLP-1 group increased significantly compared with the control group under morphological observation.The expressions of specific genes [uncoupling protein 1 (UCP1),cell death-inducing DFFA (DNA fragmentation factor a)-like effector a (Cidea),peroxisome proliferator-activated receptor γcoactive 1α (PGC1-α)],differentiation genes [PRD1-BF1-RIZ1 homologous domain containing 16 (PRDM16),peroxisome proliferators-activated receptor-γ(PPAR-γ)],nitric oxide pathway genes [endothelial nitric oxide synthase (eNOS),inducible nitric oxide synthase (iNOS)] in brown adipocytes in GLP-1 group were significantly increased as compared with the control group,F values were 17.36,29.57,66.76,13.78,4.14,105.47,346.57 respectively(all P＜0.05).Conclusions GLP-1 receptor agonist may improve the differentiation and function of brown adipocytes in mice,thereby enhance the function and induce the differentiation of brown adipocytes,the effect is more evident in high concentration of GLP-1. Key words: Glucagon-like peptide-1 ; Brown adipose tissuse; Exenatide; Uncoupling protein 1 ; White adipose tissue ; Nitric oxide synthase