Abstract
Objective To study expression of integrin and epidermal growth factor in sterile patients during endometrial implantation window and discuss the relationship between the integrin and epidermal growth factor and endometrial receptivity. Methods: The research objects were divided into 5 groups with 30 women at the normal gestation age, 30 women exhibiting ovulation following PCOS ovulation induction treatment, 26 women exhibiting no ovulation following PCOS ovulation induction treatment, 38 women exhibiting oviduct obstruction, 32 women exhibiting hydrosalpinx. An immunohistochemical method was used to detect the expression of integrin and EGF in sterile women of the five groups of research objects during endometrial implantation window and a statistical analysis was conducted for all detection results. Results The expression level of the integrin in PCOS group is significantly lower than that in the control group and the difference is significant (P<0.05); the expression level of the integrin in PCOS anovulation group is significantly lower than that in PCOS ovulation group and there is statistical significance (P<0.05); the difference between the oviduct obstruction group and the control group is not significant (P>0.05); the expression level of the integrin in the hydrosalpinx group is lower than that in the oviduct obstruction group and the difference is significant (P<0.05). EGF is expressed in endometria of various groups of women and its expression is located in cytoplasm of epithelial cells of the endometrial gland. Conclusion Integrin and epidermal growth factor are expressed in endometria of various groups of women in the implantation phase indicating that they may be important regulatory factors for endometrial receptivity and embryonic implantation.
Highlights
In recent years, reports both at home and abroad have shown that infertility morbidity is increasing gradually
The endometrium expresses specific genes and combines specific protein enabling the endometrium to receive embryonic nidation [5]. These proteins can serve as biochemical markers for Science Journal of Public Health 2020; 8(2): 56-62 endometrial receptivity and clinical detection of such markers helps determination of the implantation window phase
Ltd.; Bovine Serum Albumin was purchased from Shanghai Jianglai Biotechnology Co., Ltd.; rabbit anti human epidermal growth factor (EGF) polyclonal antibody and rabbit anti human integrin monoclonal antibody were purchased from Shanghai Boyan Biotechnology Co., Ltd.; S-P hypersensitivity reagent kits were purchased from Beijing Ruixiang Biotechnology Co., Ltd.; Model XSZ-D2 inverted biological microscope was purchased from Japan MuPid Company; the full-automatic chemiluminiscence immunoassay analyzer was purchased from U.S Thermo Company; the multifunctional microplate reader was purchased from U.S Labnet Company; the low-temperature ultra centrifuge was purchased from Beijing Jingli Centrifuge Co
Summary
Reports both at home and abroad have shown that infertility morbidity is increasing gradually. The pregnancy success rate of sterile women still remains at 35%-40% and the bottleneck can not be broken through at all times the assisted reproductive technology is maturing increasingly. A normal endometrium only allow for embryonic nidation in a very short period In this period, the endometrial receptivity is the greatest and it is usually referred to as "implantation phase" [3]. The endometrium expresses specific genes and combines specific protein enabling the endometrium to receive embryonic nidation [5]. These proteins can serve as biochemical markers for Science Journal of Public Health 2020; 8(2): 56-62 endometrial receptivity and clinical detection of such markers helps determination of the implantation window phase
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