Abstract
Cell counting has a wide range of applications in cytology research and clinical practice. Cell counts are widely used in the early diagnosis and treatment of serious diseases and the screening of effective drugs in the later stage. The method of studying cell counts is of great significance. This paper proposes a method for cell counting based on the highlight area of the cell center as a live cell marker. This method uses algorithms bilateral filtering to smooth noise in the original image; after adaptive threshold segmentation, an algorithm based on flood filling is used. Accurate segmentation of the highlighted area in the center of the cell; finally, counting is achieved through the connected domain labeling algorithm. The experimental results show that the accuracy of the cell counting method proposed in this paper is above 98%, which verifies the feasibility and practicability of the method proposed in this paper.
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