Research on auto focusing algorithm of microscopic imaging system

Abstract

In the intelligent microscopic imaging system, the focusing evaluation function is one of the important core links in the automatic focusing system. In order to solve the problem that the focusing curve loses the characteristics of the ideal curve caused by illumination change, an automatic focusing algorithm based on Robert and wavelet transform is proposed in this paper. The algorithm extracts the maximum value of the high-frequency component wavelet coefficients decomposed by the wavelet transform, and uses the extracted wavelet coefficients to weight the Robert evaluation value coefficients, increases the sensitivity factor of the algorithm, and makes an experimental analysis with five traditional focusing functions under the condition of different illumination intensity to verify the feasibility of the algorithm. The experimental results show that the algorithm can overcome the change of illumination, has strong anti noise, sensitivity and stability, and is suitable for the fine focusing process in the microscopic imaging system.